However, research is not considered conclusive. ; "Manufacturing Economies and Product Improvement Through Advanced Freeze Drying Techniques"; Reprint of talk to Annual Meeting of Parenteral Drug Assn. Bacteriostatic water is a sterile form of water that has been brought to a pH between 4.5 to 7.0. Failure to do so risks the development of hemolysis, damaging the red blood cells [9, 10]. Bacteriostatic Water for Injection, USP must be made approximately isotonic prior to use. Dont just shoot the water like a spray or something. The safety of the plastic has been confirmed by tests in animals according to USP biological standards for plastic containers. Once a vial of bacteriostatic water is opened, the benzyl alcohol will usually prevent the growth of bacteria for up to 28 days. Obviously, a low fill would represent a subpotency in the vial. Acidic peptides will be best soluble in basic solution and vice versa. publishing any Website material in any other media; selling, sublicensing and/or otherwise commercializing any Website material; publicly performing and/or showing any Website material; using this Website in any way that is or may be damaging to this Website; using this Website in any way that impacts user access to this Website; using this Website contrary to applicable laws and regulations, or in any way may cause harm to the Website, or to any person or business entity; engaging in any data mining, data harvesting, data extracting or any other similar activity in relation to this Website; using this Website to engage in any advertising or marketing. Immediately you can gauge which is appropriate based on allergies. It depends on the compound, there is no universal conversion, About: Muscle and Brawn is a coaching company specializing in bodybuilding, hormones, peptides, anabolics, and other biohacking. First, lets break down the basics of sterile water. Reconstitution follows the above method. Bacteriostatic water is water with benzyl alcohol. The small volume of fluid provided by Bacteriostatic Water for Injection, USP when used only as a pharmaceutic aid for diluting or dissolving drugs for parenteral injection, is unlikely to exert a significant effect on fluid balance except possibly in very small infants. swelling of your face, lips, tongue, or throat, redness, warmth, and pain at the injection site, tenderness or irritation at the injection site. On the insulin syringe, 100 units correspond to 1ml, so 10 units will correspond to 0.1ml of the bacteriostatic water. Both bacteriostatic water and sterile water are not used for straight injections, they have to be diluted with a drug or solvent. During peptide reconstitution, two variations of solutions are popular. It is not intended nor implied to be a substitute for professional medical advice. Reactions which may occur because of this solution, added drugs or the technique of reconstitution or administration include febrile response, local tenderness, abscess, tissue necrosis or infection at the site of injection, venous thrombosis or phlebitis extending from the site of injection and extravasation. So, if you are someone who has seen the vials of both types of water, I am sure you can now understand why a bacteriostatic water vial contains considerable volume while a sterile water vial contains just a few milliliters. Slowly extract the bacteriostatic water until the solution reaches your desired level. This Website is provided as is, with all faults, and Buckle LLC express no representations or warranties, of any kind related to this Website or the materials contained on this Website. Mrose . Medically reviewed by Drugs.com. Leucovorin Calcium - GlobalRPH If you are a peptide researcher or laboratory professional, you can safely enjoy the benefits of bacteriostatic water with proper use. The struggle is real, people. Bacteriostatic water has wider availability and is simpler to manufacture. Because of the benzyl alcohol contained in Bacteriostatic Water for Injection, USP, when doses greater than . Once the peptide is really made soluble in any of the relevant solutions, diluting it with bacteriostatic water or sterile water is recommended. 420 Throckmorton Street, Suite 200, Fort Worth Texas 76102, Copyright 2022 BacteriostaticWater.com - All Rights Reserved, Wholesale Hospira Bacteriostatic Water Supplier, Need help? That means, they are single-dose vials and should be discarded after a single-use. How To Reconstitute PT-141 In Bacteriostatic Or Sterile Water Well, people have been mixing peptides in the same syringe and then administering them. Selective Estrogen Receptor Modulator (SERM), Induction of Ovulation with CLOMIMED 50 (Clomiphene Citrate), Anemia in Cancer Patients: treatment with DECAMED 250 (Nandrolone Decanoate), PROVIMED 25 (Mesterolone) for the Treatment of Male Infertility. 5.0 out of 5 stars Good product. Many of the antibiotics, such as some of the semi-synthetic penicillins, cephalosporins, and also some of the salts of erythromycin, doxycycline and chloramphenicol are made by the lyophilization process. 3, pp. Specialists generally consider bacteriostatic water to be a more efficient solvent or dilutant. Because they are antibiotics, low bioburden of these formulations would be expected at the time of batching. Additionally, stability data should include provisions for the assay of aged samples and subsequent reconstitution of these aged samples for the maximum amount of time specified in the labeling. By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. On the other hand, distilled water is rather more sterilized and contains neither organic nor organic materials. FDA indications vary regarding drug interactions. My recommendation is to use bacteriostatic water as that's what most peptide vendors sell. Do NOT reuse the same saline syringe to flush VADs before and after medication administration. In case you have any issue diluting peptides in bacteriostatic water, you should add a small amount of 10% aqueous acetic acid for basic peptides and aqueous ammonia for acidic peptides. All you have to do is just follow the easy steps and BOOM! event these should occur, re-evaluate the patient and institute appropriate Side Effects of Bacteriostatic Water (Bacteriostatic Water for - RxList Once a vial of sterile water is open, it should be used completely at once or used once and discarded. Handlers should adhere to the exact indications or medical instructions. With our easy-to-use reconstitution calculator, you now dont have to worry about all the multiplication, division or the unit conversion and all. Here are some general guidelines for storing reconstituted peptides: Short-term storage: If you plan to use the reconstituted peptide within a few days, you can store it at 4C in the dark. PHARM TEST #2: Reconstitution Flashcards | Quizlet As mentioned previously, sterile water only has distilled water and nothing else. The pH is 5.7 (4.5 to 7.0). Bacteriostatic Water The process of reconstitution is the same regardless of what kind of water you chose to reconstitute your AOD with. If an adverse reaction does occur, discontinue the infusion, evaluate the patient, institute appropriate countermeasures, and if possible, retrieve and save the remainder of the unused vehicle for examination. Before sharing sensitive information, make sure you're on a federal government site. Experts state that it may be necessary to run trials in order to select the best solvent for various peptides, and sodium chloride water should be avoided [12]. On the other hand, a sterile water vial doesnt contain any preservatives/additives which can keep it free of pathogens for a longer time, thus one will have to use it immediately after opening the seal. Some of the older lyophilizers cannot tolerate steam under pressure and sterilization is marginal at best. Because visual guidance is far better to approach, I searched for some of the Youtube videos that could help you in the process. It could be potentially harmful to try to reconstitute a peptide meant for mixing with bacteriostatic water with sterile water. Some peptides are still off-limits to us mere mortals, and only available for research purposes. This discussion will address some of the problems associated with the manufacture and control of a lyophilized dosage form. Example would be 5g of CJC-1295 and 5g of ipamorelin. Reconstitution Solution versus Bacteriostatic Water. The action you just performed triggered the security solution. The clinical applications of the hormone hCG include fertility enhancement in both women and men, as well as a supplement to TRT in male subjects [17, 18]. Bacteriostatic water and sterile water are essentially used for the same function which is to dissolve the drugs/medications for injecting purposes. Parenteral preparations containing benzyl alcohol should not be used in epidural or spinal anesthesia procedures. BACTERIOSTATIC 0.9% SODIUM CHLORIDE INJECTION, USP 10mL VIAL RedwolfWV Registered User Awards 1 May 24, 2008 #4 Ahh I get it. This parenteral preparation is unlikely to Using either in the proper conditions can provide extraordinary results. Another key difference is availability. The pH is 5.7 (4.5 to 7.0). Create your account and enjoy a new shopping experience. I recommend using bacteriostatic water since that's what the peptide vendors sell and recommend. See WARNINGS, PRECAUTIONS Bacteriostatic water is a sterile form of water that has been brought to a pH between 4.5 to 7.0. Another difference is indications. Bacteriostatic Water vs "reconstitution solution" what's - Reddit I'm diluting my AA (vinegar) to get to 0.6% AA. What is bacteriostatic water? Bacteriostatic water (BW) has a pH of about 5.7. Read more. The re-use of sterile water for injections is strictly prohibited. It also is prepared with a bacteriostat, a compound used to prevent any bacterial growth. They both are USP meaning they have an official monograph or documentation by the United States Pharmacopeia, both are not used for IV solutions. Average normal adult daily requirement ranges from two to three liters (1.0 to 1.5 liters each for insensible water loss by perspiration and urine production). If kept under appropriate conditions, bacteriostatic saline can last up to 28 days. 11, pp. Reconstituted peptides can be stored in several ways, depending on the duration of storage and the desired shelf life. If any provision of these Terms is found to be invalid under any applicable law, such provisions shall be deleted without affecting the remaining provisions herein. (WITHOUT GRABBING YOUR FREE COPY OF THE SARMS AND PEPTIDES SECRETS MINI COURSE). If you feel that any of our content is inaccurate or out-of-date, please contact us at: [emailprotected]. 1) Determine the quantity of peptide, in micrograms (mcg), in your vial. Not only is this inefficient, but you may come across low-quality products from inferior manufacturers. A generally recognized acceptable method of sterilizing the lyophilizer is through the use of moist steam under pressure. It may be wise to always opt for bacteriostatic water if both water types are allowed for use. 1. : the action of reconstituting or state of being reconstituted. Research peptides present great potential in emerging clinical contexts, such as combating symptoms of cardiovascular and metabolic diseases, as well as improving reproductive health [11]. It is also not known whether Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection containing additives can cause fetal harm when administered to a pregnant woman or can affect reproduction capacity. Data are unavailable on the toxicity of other preservatives in this age group. Bacteriostatic 0.9% Sodium Chloride (NaCl) Injection, USP is a sterile, nonpyrogenic, isotonic solution of sodium chloride in water for injection. Benzyl alcohol can be a topical irritant but in the concentration of 0.9% does not typically cause irritation and may provide some anesthetic effects. (Isotonic saline = 0.28 osmolar.) Sterile water is free of any organic materials but contains inorganic materials to some extent. The dosage and administration schedule should follow applicable recommendations [18, 19]. Do NOT use sterile water for injection for flushing VADs. In summary, the best way to store reconstituted peptides depends on the duration of storage and the desired shelf life. FDA rev date: 6/15/2000. Water is an essential constituent of all body tissues and accounts for approximately 70% of total body weight. We strive to never have a piece of inaccurate information on this website. If the peptide still doesnt get dissolved, perform trial and error and use various other solvents. Our content is continually monitored by an internal peer-review process to ensure accuracy. Water balance is maintained by various regulatory mechanisms. These lyophilizers can only have their inside surfaces wiped with a chemical agent that may be a sterilant. You can go as high as 2 milliliters per peptide reconstitution as it does no harm other than more dilution. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. Bacteriostatic Water for Injections | The A-Z Guide, Add a header to begin generating the table of contents. This is one of the reasons that vials of sterile water usually contain a few milliliters of the liquid. All you have to do is add the desirable reconstituted peptides in 1 vial and then use the syringe to pull out the mixture so that you can administer it. tion ()r-kn (t)-st-t (y)-shn. Also, nothing contained on this Website shall be interpreted as advising you. For intravenous or intramuscular injection, prepare solution by aseptically adding not more than 2 mL of Bacteriostatic Water for Injection or Bacteriostatic Sodium Chloride Injection to the contents of one vial. In the First, you want to thoroughly wash your hands with soap and water for at least 20 seconds. But, for long-term storage of the powder form, the required temperature is less than -20 degrees celsius. Most peptide packages contain mixing instruction. Performance & security by Cloudflare. Use only as a diluent or solvent. Buckle LLC reserves the right to remove any of Your Content from this Website at any time without notice. For example, the recommended dosage of BPC-157 is 1mcg to 10mcg per kg of body weight which accounts for 200mcg to 1000mcg per injection. RxList does not provide medical advice, diagnosis or treatment. Both meet US Pharmacopeia (USP) standards. Always double-check which mixture you have because you cannot interchange between these two formulations. Bacteriostatic water can be used multiple times for up to 28 days before the suggested disposal. It is only used for injections such as intravenous, intramuscular and subcutaneous. Vaxxen Labs Reconstitution Solution (30 mL) 2 Pack So, if you want to maximize the efficacy of your peptides, follow our guide step-by-step, and soon youll be a reconstitution pro. How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? Additionally, bacteriostatic water must always be mixed with a solute before injection. This can come into play when preparing for traveling, budgeting monthly expenses and storage. Unfortunately, piping such as that for the administration of inert gas (usually nitrogen) and sterile air for backfill or vacuum break are often inaccessible to such surface "sterilization" or treatment. New comments cannot be posted and votes cannot be cast. and ADVERSE REACTIONS. Do not use Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection, USP for intravenous injection unless the osmolar concentration of additives results in an approximate isotonic admixture. When necessary, the pH of each formula was adjusted with sodium hydroxide so that the pH of the reconstituted solution is within the USP specified range of 7 to 8 and the tonicities are, for the 40 mg per mL . Muscle + Brawn 2023. Inspect reconstituted (diluted or dissolved) drugs for clarity (if soluble) and freedom from unexpected precipitation or discoloration prior to administration. Lyophilized peptides are considerably stable at room temperature, so if you dont want to reconstitute the lyophilized peptide for some time, dont worry and just keep it a room temperature. Reconstitute Definition & Meaning - Merriam-Webster Plus, peptide solutions made with bacteriostatic water resist contamination. The semi-rigid vial is fabricated from a specially formulated polyolefin. It is recognized that there is complex technology associated with the manufacture and control of a lyophilized pharmaceutical dosage form. Do NOT dilute or reconstitute medications by drawing up the contents into a commercially available, prefilled flush syringe of 0.9% sodium chloride and then administering the resultant product. 100 mg Plain. Water balance is maintained by various regulatory mechanisms. One vial of BPC 157 contains 5mg, which also means 5000mcg. Please refer to the WARNINGS Both may be used in the injection of peptides, hormones, and various medications, often reconstituted from lyophilized powder. The Buckle LLC is allowed to assign, transfer, and subcontract its rights and/or obligations under these Terms without any notification. This significantly reduces the risk of infection [, It is an efficient solvent and presents minimal interaction with most medications [, Apart from medications, bacteriostatic water is also indicated for the reconstitution and injection of therapeutic research peptides and hormones [, Suitable injection routes are determined by the type of solute, the recipients needs, and target rates of diffusion and metabolization. Bacteriostatic Water for Injection: PI - Drugs.com Although products may be labeled for reconstitution with Bacteriostatic Water for Injection, Sterile Water for Injection (WFI) should be used to reconstitute products. For more information, please see our Once vials are partially stoppered, they are transported to the lyophilizer. The next step is to do is add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the x ml of bacteriostatic water in order to get your desirable dosage in mcg. Carefully install the needle tip on a syringe. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials. But not all peptides dissolve in water easily, so it usually is recommended that you try to dissolve the peptide in any of these waters using Sonication. We focus on finding the most accurate information from the scientific source. Providing a method for introducing the sterile moisture with uniformity may be difficult. Why is Water Essential to Reconstitution? For the 5mg vial of BPC 157, you will have to use 1ml of bacteriostatic water. The following preparation is designed solely for parenteral use only after addition of drugs that require dilution or must be dissolved in an aqueous vehicle prior to injection. No information provided. Based on 31 documents. Curious about Bacteriostatic Water for injections? It is considered an ideal sterile solution due to its low toxicity and minimal interference with drug effectiveness. What is the difference between bacteriostatic water and sterile water This monograph has been modified to include the generic and brand name in many instances. It also is prepared with a bacteriostat, a compound used to prevent any bacterial growth. Sample 1 Sample 2 Sample 3. If I have a 2mg bottle how much water total do I put into the vial? Some drugs can interact poorly with each solvent, and they are not considered universally interchangeable in clinical contexts. Many people have this mentality that bacteriostatic water and sterile water are the same thing, and that can bring disastrous results. This method can preserve the peptides stability and activity for several months to years, depending on the peptides properties and storage conditions. Bacteriostatic water vs. sterile water: Reconstitution Bacteriostatic water is in many cases the best solvent or dilutant for injections of medications and other therapeutic substances, such as peptides and hormones. It is only used for injections such as intravenous, intramuscular and subcutaneous. They both are nonpyrogenic meaning they wont cause fever. Recent inspections have disclosed potency and sterility problems associated with the manufacture and control of lyophilized products. So, the former is available in multi-dose containers that are viable for up to 28 days after opening, while sterile water comes in single-dose vials. If you dont know what BPC 157 is, it is a peptide that is used by athletes and bodybuilders to recover from injuries and aid in the healing process. Lets nail the major differences right from the start. 51.222.156.250 IMPORTANT Use only the accompanying diluent or Bacteriostatic Water For Injection with. What Is Bacteriostatic Water For Peptide Reconstitution But dont get too excited just yet. However, they do differ in their composition as the bacteriostatic water contains 0.9% benzyl alcohol as a preservative. Due to the pH, a more apt solution for fluid replacement is normal saline or dextrose. Although products may be labeled for reconstitution with Bacteriostatic Water for Injection, Sterile Water for Injection (WFI) should be used to reconstitute products. It is simply water that has gone through purification and distillation processes for its safe use in various therapeutic contexts, namely injections, dilution, and irrigation. SIDE EFFECTS. This process is often helpful in breaking down the big solid lumps of peptides in the water and aids in the dissolution process. Intravenous administration of Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection without a solute may result in hemolysis. Plus, youll feel like a mad scientist, and who doesnt love that feeling? ], Hospira, Inc., Lake Forest, IL 60045 USA. The concern with any microbiological level is the possible increase in endotoxins that may develop. a. There may be cases in which sterile water is more appropriate, as outlined above. PDF SOLU-MEDROL (methylprednisolone sodium succinate for injection, USP The 3) Choose the size of the syringe you want .
Mia Costa Pioli, Articles I